This article presents the measurements of indoor climate in classrooms in the same school in Denmark. The classrooms had different ventilation systems: Ventilation was achieved either by manually operable windows, or by automatically operable windows with and without an exhaust fan in operation, or by a balanced mechanical ventilation system. Indoor air temperature and carbon dioxide (CO2) concentration, as well as opening of windows were continuously monitored for one month in the non-heating and heating seasons; measured CO2 concentration was used to estimate average classroom ventilation rates. The results show that mechanical ventilation and natural ventilation with automatically operable windows with exhaust fan performed notably better than the other systems. They indicate also that opening of windows was largely affected by customs and habits. Present results can be used as the basis for rational selection of systems that ensure adequate classroom ventilation.